A study was carried out to determine the effect of dietary feed additives such as organic acid (Poulacid), probiotic (Lacprobi) and β-glucan (Beta-glucan 25) supplementation to diet on performance and egg quality of laying hens at the early of laying stage. Three hundred and eighty four (384) Isa Brown laying hens at the 21 weeks of age were randomly distributed in a completely randomized design experiment, with 4 treatments and 24 replicates (each replicate consisted of 4 birds/pen). Treatment control (ĐC): Basal diet without supplementation of any feed additive; OAD: Basal diet + 0.1% organic acid;  PBD: Basal diet + 0.1% probiotic; BGD: Basal diet + 0.1% β-glucan. The results showed that no effect of feed additives on feed intake and egg weight, but a little improvement hen day production and egg mass  in OAD (81.7% and 41.2 g/bird) PBD (81.5% and 41.5 g/bird)  compared to  control (78.3% and 39.6 g/bird) and BGD (79.4% and 40.1 g/bird). The OAD and BGD diets tended lower proportion of broken, unnormal and double york eggs compared with the control group. There were no significant effect of treatments on egg shape index, albumen weight, yolk weight, shell weight và Haugh Unit. But there was a light improvement of shell thickness and york color in OAD (0.401 mm and 10.58) and BGD (0.403 mm and 10.21) groups compared with  control (0.391 mm and 9.51) and PBD (0.394 mm and 10.3). It could be concluded that the supplementation of organic acid and probiotic at 0.1% caused improvement of hen day production, FCR and egg york color, and increased egg shell thickness in hens fed organic acid and β-glucan supplementation in the diets compared with control.