The study entitled “A survey on fish species ratio at Tram Chim National Park, Tam Nong district, Dong Thap province” was conducted from October 2019 to August 2019 with the objective to assess the fish species composition within the A1 zone of Tram Chim National Park. The study identified 92 species of freshwater fish species coming in and out of the park. The time period when the most species appeared during the survey was from 12am to 6pm with the white fish group always prevailed over the black fish group.