This paper, based on document research, field surveys and questionnaires, aims to analyze the factors which are influencing the development of ecotourism at An Binh islet, Vinh Long province, Vietnam. Through Exploratory Factor Analysis, the findings reveal that there are 9 factors affecting the development of ecotourism at An Binh islet namely Tourism resources, Infrastructure and Technical facilities for tourism, Human resources, Food and beverage services, Service prices, Security - order and safety status, Tourism promotion, and Environmental interpretation education. This study also proposes some solutions to increase quality of ecotourism and encourage more and more tourists to An Binh islet, Vinh Long province. Thanks to the finding of study, the result will benefit local authorities, management, tourist agencies, ecotourism business owners and local people with real data analysis.