The study was done through interview of 60 white-leg shrimp farmers in Binh Dai and Thanh Phu District, Ben Tre province in order to investigate the chemical use, disease occurrence and farming practice. Results showed that the most common shrimp disease are AHPND (56.7% and 63.3%) and white feces disease (50 and 63.3%), reported by farmers. The commonly used antimicrobials were amoxicillin (20-30%), cotrim (16.7-26.7%) and oxytetracycline (10-26.7%). The used antimicrobials were belonged to the allowed listed issued by Ministry of Agriculture and Rural Development, Vietnam. Most of farmers in both models using product containing chlorin as disinfectant in water input ponds while iodine and BKC were used during grow-out. Nearly all farmers used probiotic for digestive enhancement and water quality improvement, common species were Bacillus subtilis, B. licheniformis, B. megaterium. However, the effectiveness was unknown. Thus, there is an urgent need to provide training to shrimp farmers to enhance knowledge on effectiveness use of chemicals, guarantee the food safety for the final product