HDAC enzymes play important role in expression of many human diseases (including diseases can cause death), therefore it is necessary to profound study HDAC enzymes and their inhibitors. Nowadays, using in silico methods (method of using computer) in research and development medicines becomes common. So in this study, we apply AutoDock software to show interaction between romidepsin and HDAC6, HDAC8 enzymes. And the docking results based on theoretical calculations showed that romidepsin could not bind to Zn2+ ion, but it could bind to many major amino acids in the active region of HDAC.