This study aimed to evaluate effects of broodstock populations on growth and survival at different life stages of climbing perch offspring. Broodstock were collected from four populations including (i) square head (cultured) population and 3 wild populations sampled in (ii) Ca Mau, (iii) Dong Thap, and (iv) Hau Giang provinces (in the South of Vietnam). One-day old fry randomly sampled with the same portions from 5 families of each populations were mixed before stocking. Experiments included three stages: (1) fry nursing for 14 days; (2) fingerling rearing from 15 - 55 days old, and (3) grow-out from 2-6 months old. At the first stage, fry were stocked randomly in 16 100L-tanks (200 individuals/tank) and fed with live food (Rotifer, Moina) combined with commercial feed (42% protein). At the second stage, fish were reared in two 16-tank systems (random block design) including 1m³- and 200L- tanks with the same density of 500 fish/m³, and fed with commercial feed (40% protein). At the grow-out stage, 100 individuals with uniform size fingerlings were randomly assigned to one of 16 hapas (2.25 m³) suspended in the same pond. Difference in initial sizes within treatments was treated as block effect.