Research of the effects of brassinolide (BL) on the growth and yield of sesame, the field experiment was carried out in Randomize Complete Block design (RCBD) with 5 treatments and 5 replications, the replication area is 65m2. The treatments were three concentrations of BL (0.05, 0.07 and 0.1 ppm), and 2 control treatments, Ca- Bo- K (CaO (18 mg/l), K2O (144 mg/l) and B2O3 (384 mg/l) (as farmer using) and spray water. All treatments were treated at two times, 15 and 30 days after sowing. The results showed that plants treated with BL, the proline content in leaves increased. Sesame was sprayed with 0.07 ppm of brassinolide producing highest yield (1,637.7 kg/ha) (increasing double times and 34.9% in comparison with spraying water and farmer control treatment, respectively).