A digestibility experiment was conducted to determine the effects of Catfish by-product meal (CT) replacement at 0, 50 and 100% of sea fish meal (CB) with and without Probiotic (M) suplementation in the diets for cross breed (Noi x Luong Phuong) chickens. The experiment was conducted with 24 male chickens at 8 weeks of age and were placed in individual pens to collect excreta. Six treatments (CT0, CT0M, CT50, CT50M, CT100, CT100M) were introduced to determine the apparent ileal (AID) and total tract (ATD) digestibility of nutrients and amino acids using Chromic oxide (Cr2O3) as indigestible marker.After 4 days of colleting excreta, the chickens were killed to collect the ileal digesta.The AID and ATD of crude protein (CP) decreased with increasing CT in the diets (Pand ATD digestibility.There were significantly reduce (Pisoleucine, leucine, lysine and ileal digestibility of arginine, isoleucine, leucine, lysine and methionine when increasing CT inclusion on the diets. The chickens fed diets with probiotic supplementation increased the nutrients and amino acids digestibility of both AID and ATD.