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Bài báo - Tạp chí
4(11) (2015) Trang: 309-314
Tạp chí: INTERNATIONAL JOURNAL OF ENGINEERING SCIENCES & RESEARCH TECHNOLOGY
Liên kết: www.ijesrt.com
Optimization the extraction process of flavonoids  from soybean seeds (Glycine max L.)  by response surface 
methodology (RSM) in conjunction with central composite design (CCD) was performed. The maximum yield of 
total flavonoids content (TFC) and the 2,2-diphenyl-1-pierylhydrazyl (DPPH) free-radical scavenging potential were 
used as quality indicators. The extraction was carried out three cycles by using acetone as solvent. The range of the 
independent variables, namely acetone concentration (60–80%, v/v), the ratio of solvent and material (6:1–10:1, 
v/w), the extraction temperature (30–50°C) and time for each cycle (180–240 minutes) were identified by a first set 
of single and two factor experiments. The optimum conditions for extraction were found to be at acetone 
concentration 69%, the ratio of solvent and materia l 8:1 (v/w), extraction temperature 42°C and 184 minutes for 
each cycle extraction. Under these optimized conditions, the experimental yields of TFC and DPPH free -radical 
scavenging potential were respectively 2.2 ± 0.01 mg QE/g DW and 79.49 ± 0.16% which  were in close agreement 
with predicted values, This indicates the suitability of the models developed and the success of RSM in optimizing 
the extraction conditions.
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