The business environment always contains opportunities that affect almost the entire entrepreneurship process. Several scholars have been researching how to take advantage of opportunities in creating a business.  This study analyzes determinants of the exploration, business opportunity exploitation, and entrepreneurial intentions of 447 people who have never started a place in the Mekong River Delta. Based on the opportunity-based theory and applied the Structural Equation Model (SEM) results, we find strong evidence that opportunity exploration positively impacts opportunity exploitation. At the same time, opportunity exploitation has a positive effect on entrepreneurial intention. This study aims to arouse the people's entrepreneurial spirit in particular and support the policymakers in devising the locality's entrepreneurship policy in general.