In order to detect and type of porcine parvovirus (PPV), PCR assay was developed and applied on porcine lung samples in this study. Each of target genes of PPV1, PPV2 and PPV3 was amplified using the specific primers. Total of 146 lung/blood samples collected from several slaughter houses belonged to seven provinces of central part of Vietnam were tested, which are Quang Binh, Quang Tri, Thua Thien Hue, Quang Nam, Da Nang, Quang Ngai and Binh Dinh. Overall prevalence of singular PPV1, PPV2 and PPV3 were 52.7% (77/146), 56.2% (82/146) and 5.5% (8/146), respectively. The results also showed that the PPV1, PPV2 and PPV3 were co-circulated while the PPV1/PPV2 co-infection was predominant of 34.2% (50/146). The rates of co-infection of PPV1/PPV3 and PPV2/PPV3 were similarly lower with 4.1 and 4.8%, respectively. Only 3.4% (5/146) of pigs was positive with three genotypes PPV1/PPV2/PPV3. The prevalence of PPV genotypes was consistent with sequencing results. It proved that convenient PCR assay could be used as a differential diagnostic tool for monitoring and control of PPVs in the field. The result of this study confirmed the circulation of the PPVs infection in pigs in central Vietnam.
Tạp chí khoa học Trường Đại học Cần Thơ
Lầu 4, Nhà Điều Hành, Khu II, đường 3/2, P. Xuân Khánh, Q. Ninh Kiều, TP. Cần Thơ
Điện thoại: (0292) 3 872 157; Email: tapchidhct@ctu.edu.vn
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