The fish resource assessment has strongly related to their growth pattern estimated from fish lengthweight relationship and condition factor, but these data on Acentrogobius viridipunctatus—a traded gobies in the Mekong Delta, Viet Nam have been limited. Consequently, the present study was performed for a year-round 2020 in order to provide knowledge on the length-weight relationship (LWR), growth and condition factor (CF) of this fish species. A total of 960 fish (432 females and 528 males) were caught at four coastal sites from Long Huu, Tra Vinh to Tan Thuan, Ca Mau. Due to high determination values of LWRs (r2 > 0.8), the fish weight could be estimated from a given fish length. Since the slope value (b = 2.64 ± 0.03) of LWR was