This study provides new knowledge of length-weight relationship (LWR), growth pattern and condition factor (CF) of Butis humeralis, one of target catching gobies for food supply in the Mekong Delta, based on a collection of 1,439 fish (580 females and 859 males). Gill nets are used to catch fish samples for twelve months, from April 2019 to March 2020, at six sites in estuaries in the Mekong Delta. The analyses show that the weight of males and females of different fish sizes, seasons and sites are determined by a given total length since the determination coefficients of LWRs are high (r2>0.8 for all cases). As the slope coefficient values (b) are higher than the threshold of three, both males and females display positive allometric growth patterns. Likewise, fishes caught at two fish sizes in two seasons and five sites (except in Vinh Hau, b=2.97±0.12 SE) exhibit positive allometry. The CF of Butis humeralis fluctuates around the well-being parameter of one, ranging from 0.95±0.01 SE to 1.08±0.02 SE, which indicates that these six sites are suitable environments for fish growth. The results yield reliable biological data for further research on manageable and conservable strategies of this species.