The aim of the study was a preliminary determination of occurrence of extended spectrum beta-lactamase producing Escherichia coli (ESBL E. coli) in chickens collected from slaughter-houses located in different regions in the Mekong Delta of Vietnam. A total of 720 samples from 180 chickens were tested, comprising 180 liver samples, 180 lung samples, 180 meat samples, and 180 fecal samples. Isolated and identified E. coli strains were examined for the ESBL production by the use of combination disc methods.  The presence of the CTX-M, TEM, and SHV genes were examined by Polymerase Chain Reaction method. The results showed that 48.33% chickens contained ESBL E. coli; the presence of ESBL E. coli on liver, lung, meat, and fecal samples were 5%, 11.67%, 11.11%, and 48.33% respectively. The presence of the genes atAll isolates showed the presence of the  antibiotic resistance genes including 87.5% CTX-M gene, 75% TEM gene, and 70% SHV gene.