Bacterial leaf blight (Xanthomonas oryzae pv. oryzae) is one of the most serious diseases in rice fields. Climate changes are attributed to increase virulence of the pathogen as this was shown to be correlated with the increase of temperature. The Plant Pathology Research Group at the Biotechnology R&D Institute of Can Tho University has worked on biological control of this disease using antagonistic microorganisms and plant extracts to diminish the use of chemicals. This paper presents the research results of this group on the use of antagonistic soil bacteria, i.e., isolation, testing for the antagonistic and disease-reducing effects under both greenhouse and field conditions, identification, biomass production and selection for carrier materials to make bio-formulations of the antagonistic bacteria.