Article info.

ABSTRACT

Received date: 20/10/2015

Accepted date: 30/11/2016

In this study, we compared fungal population between the rice monoculture and the polyculture on fungal population. The studied crop rotation systems were rice-rice-rice (CRS1), rice-rice-baby corn (CRS2), rice-rice-mungbean (CRS3) and baby corn-rice-mungbean (CRS4). A fungal primer pair (ITS1–ITS2) was used to amplify internal transcribed spacer (ITS) sequences from total DNA extracts from rice straw residues. Amplified DNA was analyzed by DGGE. Individual bands from DGGE gels were cloned and sequenced. DGGE gels representing the fungal communities of the continuous rice and the rotation site were compared and related to fungal identified from the field.

The results demonstrate that the fungal communities of rice straw residues varied greatly due to the crop rotation. Fungal communities colonizing the rice monoculture were found significantly different from alternating crop of mungbean and baby corn. Cloning and sequencing of bands from the DGGE gels revealed the presence of Ascomycota and Basidiomycota that are common in agricultural soils. It was shown that the colonizing rice straw residues of crop rotation seemed to select the phylum Ascomycetes, while different members of the phylum Ascomycota and Basidiomycota were detected in the rice straw residues of the rice monoculture.

Keywords

Fungal, DGGE, rice monoculture, crop rotation, internal transcribed spacer

Cited as: Dung, T.V., Dong, N.M. and Vien, D.M., 2016. The effect of crop rotation on the structure of the fungal community colonising rice straw residues in paddy rice cultured soil in the Mekong Delta of Vietnam. Can Tho University Journal of Science. Vol 4: 52-62.