Along with the speed of industrialization and modernization, the problem of urban environmental pollution is also increasing. The study aims to assess the characteristics  of urban wastewater in Vinh Long province in 2019. The study collects data at 22 monitoring locations (denoted from U1 to U22) in Vinh Long province, Vietnam. Evaluation data is based on nine parameters including pH, temperature, TSS, BOD, NH₄⁺-N, NO₃^--N, PO₄^3--P, S^2- and coliform, which were compared with maximum values according to the guidance of national technical regulation on domestic wastewater (QCVN 14:2008/BTNMT). Pearson correlation, principal component (PCA) and cluster analysis (CA) were used in the analysis of relationship, pollution sources and key water variables resulting water quality variation, and similarities in wastewater properties in the study area. The findings showed that urban wastewater was contaminated with organic matters, nutrients, microorganisms. Correlation analysis presented that organic matters and nutrients were strongly correlated in the wastewater, with correlation coefficient ranging from 0.529 - 0.771  (TSS, BOD, NH₄⁺-N, NO₃^--N, PO₄^3--P). The PCA showed that about 88.1% of the variation in water quality was explained by 5 factors, namely living activities, market, industries, production and business establishments. CA classified properties of urban wastewater into different clusters because of organic matters, nutrients (Group IV), and microorganisms (Group I). The current study’s findings showed that direct discharge of urban wastewater will adversely affect surface water environment, so urban managers need to have solutions to treat wastewater in accordance with regulations before discharging into the environment to avoid pollution of the receiving source as well as affecting public health.