Article info.

ABSTRACT

Received 03 Jan 2019
Revised 20 May 2019

Accepted 30 Jul 2019

Lipids play important roles for the cell in storing energy, structuring cell membrane, and signaling pathway. Consequently, lipidis are analyzed routinely in various research fields. In the current research, a reliable, rapid and economical assay has been developed to quantify the total of lipid in various samples. The development of colorimetric sulfo-phospho-vanillin is for high throughput analysis of total lipids. In this method, a reaction mixture is performed in a 96-well microplate. The advantages provided from this new assay over other lipid measurement methods, included only small amount of sample requirement for fitting in the standard range (less than 100 μg/mL), less time requirement and labor in analyzing a large number of sample (about 1 hour), and the more consistent of color development between lipid content and reagent concentration.

Keywords

Measurement, sulfo-phospho-vanillin, total lipid

Cited as: Men, T.T., Thanh, N.Q.C., Yen, N.D.H., Binh, T.D. and Trang, D.T.X., 2019. A simple spectrophotometric method for quantifying total lipids in plants and animals. Can Tho University Journal of Science. 11(2): 106-110.