Four known compounds including (VC1) tricin, (VC2) apigenin, (VC3) luteolin, (VC5) luteolin-7-O-β-D-glucopyranoside and a new compound (VC4) 1α-O-methoxy-8α (4-hydroxytigloyloxy)-hirsutinolide-13-O-acetate) were isolated from the ethyl acetate partition of a ethanol extract from combined leaves and stems of Vernonia cinerea (L.) Less. Their structures were interpreted by spectroscopic methods such as ¹H-NMR, ¹³C-NMR, HSQC, HMBC, ESI-MS and based on published data.