Shrimp farming growth has led to increased use of drugs and chemicals for prevention and  treatment of infectious diseases. Pesticides have widely been used as agriculture pesticide, wild crustaceans elimination in shrimp farming, treatment of water quality and reduction of diseases. During the past 4 years, there were new agents of insecticides, fungicides and herbicides were used to protect the rice fields or integrated rice-shrimp areas. AHPNS outbreak appeared in large region and seriously affected to shrimp farming, and pesticides were found in water and sediment in these shrimp ponds. These pesticides include Deltamethrin, Fenitrothion and Hexaconazole. Environmental studies was carried out to ascertain if AHPNS pathology will develop in shrimp held in water?sediment systems containing Vibrio parahaemolyticus, the causative agent of AHPNH, and pesticides. Results showed that combination of pesticides and bacteria increased mortality of shrimp. Shrimp exposed to pesticides without bacteria did not show typical EMS/AHPNS pathology.