Can Tho City is located in the middle of the Mekong Delta, where the effects of climate change have been and will continue to be considerable. According to climate change predictions, the city is one of five regions that could be affected. In this study, we conducted analysis of trends of Tmax and Tmin over 39 years using monthly temperature in Can Tho City. We employed a Box plot, Mann–Kendall test, and Sequential Trend Analysis (SMK). Tmax and Tmin increased for all 12 months from 1984 to 2022, with Sen’s slopes of 0.45°C/decade and 0.29°C/decade, respectively. Although Tmin shows a slower rate of increase than Tmax, the increase in Tmin indicates that the temperature in Can Tho has risen.