In this study, we selected indigenous Trichoderma spp. fungi that antagonized best against Fusarium spp. fungi, which caused the pineapple stem rot disease (PSRD). The experiments consisted of the following: (i) selecting fungi causing the PSRD from rhizosphere soil and stem of infected pineapple; (ii) evaluating the control ability of Trichoderma spp. fungi selected from soils of healthy pineapple plants to the Fusarium spp. fungi. The selection resulted in 10 out of 20 Fusarium spp. fungi that caused the disease by over 20% after six weeks of infection. From 50 Trichoderma spp. fungi, the three Trichoderma strains, T. asperellum T-LM-12, T. asperellum T-LM-22, and T. hamatum T-LM-42, isolated from pineapple farms produced chitinase contents at 208.1, 243.9, and 147.2 U/mL, respectively, which were higher than those of other fungi. Furthermore, the average antagonizing indices of the T-LM-12, T-LM-22, and T-LM-42 strains against the 10 selected Fusarium spp. fungi (Fusarium equiseti strains, Fs-LM-02, Fs-LM-04, Fs-LM-07, Fs-LM-10, Fs-LM-13, and Fs-LM-14; F. incarnatum strains, Fs-LM-09; F. oxysporum strain, Fs-LM-16, and Fs-LM-18; and F. solani strain, Fs-LM-19) were 52.1%, 52.4%, and 46.2%, respectively, 144 h after of inoculation.