The study aims to demonstrate the influence of work stress on job satisfaction of employees at Can Tho Radio and Television (TV) Station. Research data were collected from 140 employees working at the Radio and TV Station of Can Tho City. Applying the structural equation modeling, the research has pointed out factors forming work stress. They include work overload, pressure from the manager, income pressure, workplace relationships, and working conditions. When work stress forms, it negatively impacts the job satisfaction of the staff at Can Tho Radio and TV Station.