The effects of temperature on the growth, survival, and digestive enzymatic activities of mud crab Scylla paramamosain were investigated in laboratory conditions. Crab juveniles (0.13 g) were exposed to four temperatures (27-28°C, 30-31°C, 33-34°C, and 36-37°C), the salinity of 25‰, and three replicates for 30 days. The density was 30 individuals per 500-L tank. The carapace width and weight in 27-28°C treatment (2.41±0.02 cm and 2.62±0.05 g) were significantly lower than those in other treatments, while the highest values were in the 36-37°C treatment (3.99±0.10 cm and 10.3±0.68 g). The digestive enzymatic activities gradually decreased with temperature rise except for amylase activity which increased up to 33°C and then decreased at the higher temperature. The molting cycle was shortest at 36-37°C (10.6±0.16 days), which was significantly lower than other treatments. Crabs reared at 36-37°C showed the highest molting frequency (2.05±0.05 times). The survival rate of crabs after 30 days was significantly reduced at the 36-37°C treatment (28.9±2.94%), compared to lower temperature treatments with the highest survival rate being 51.1±4.01% at 27-28°C. The temperature of 27-28°C was the optimal temperature for rearing this species at the juvenile stage.