From 198 actinomycetes isolated from sponges at the Kien Giang Sea in Vietnam, 42 actinomycete isolates were selected with the ability to resist Candida albicans, a species of yeast causes human diseases. There were eight isolates having strong resistance, 31 moderate, and three weak resistances. Eight best isolates were selected to identify by 16S rDNA gene analysis and sequencing. The result showed that six strains were characterized as Streptomyces spp., one strain belonged to genus Microbacterium, and one strain was genus Rhodococcus.