This study was conducted from October 2017 to March 2018 in Mo O territorial waters, Tran De District, Soc Trang province to contribute data of burrow morphology and utilization of the species Periophthalmodon schlosseri that was one of the important economical fish species in the Mekong Delta. The burrow morphology and dimensions were obtained by analyzing 30 casting burrows molded by polyester resin. The burrow utilization was determined by analyzing casting burrows and observing activities of fish in its habitats. The analysis result showed that burrows of this fish were various in shapes such as U, I, and J. The burrow had 1-2 opening with a large mound made from mud pellets, and 0-1 bulbous chamber. Dimensions of the chamber did not display seasonal and spatial changes, however, they varied with burrow shape. Furthermore, the burrow dimensional variations did not change with the interaction of season, studied sites, and shape variables. This fish used burrows as a shelter for spawning and avoiding predators. The results can be used for further research on the artificial reproduction of this species.