This study was carried out to optimize the treatment process of methyl blue (MB) in aqueous solution using dielectric barrier discharge (DBD) plasma and experiments were designed by using response surface methodology and central composite design (RSM-CCD). Four independent factors such as plasma power, liquid flow rate, air flow rate, and exposure time were investigated. According to the analyzed results of RSM, the experimental data is best fitted with a model of the quadratic polynomial with regression coefficient values of more than 0.9 for all responses. At optimal degradation conditions, the plasma power, liquid flow rate, air flow rate, and exposure time were 100 W, 1.5 lpm, 6 lpm, and 108.8 minutes respectively and the concentration of methyl blue was reduced to 95.8% with a concentration of 1.06 ppm. The degradation of methyl blue followed kinetic reaction rate was r = 1.6 10^-3[MB]^2.2.