The aim of this study was to compare biochemical traits of two rice leaf positions in induced resistance by 1,2-Benzisothiazol-3 (2H)-one 1,1-dioxide (BIT) at a concentration of 2 mM against leaf blight in rice plants. The results showed that BIT-induced rice leaves had many intense peaks which represented defensive carbohydrates, proteins and lipids. At leaves above the inoculated leaf, exogenous BIT treatment had higher peaks of lipids and proteins, such as 2920, 2851, 1736 cm-1, and the structural change of amide I from alpha helix type at the peak of 1655 cm-1 to β-sheet type at 1636 cm-1. At rice leaves below the inoculated leaf, its Fourier transform infrared peak assignments of the BIT-induced treatment had significantly spectral peaks at some vibrational peaks of lipids and carbohydrates, such as 2920, 2851, 1319, 1103 and 1040 cm-1. In this study, the elicitor of BIT reduced leaf blight severity by approximately 34.82%.