Indian pennywort (Centella asiatica (L.) Urban) is an important vegetable and medicinal herb. On the south of Vietnam, a new disease withsymptoms of bacterial blight in stems, stolons, petioles and laminasoccurred and caused severe damage at Indian pennywort fields. The purpose of this study is to identify the causal agent of the new disease and its effecton Indian pennywort at post-harvest stage. The results showed that 7 of 18 pathogen strains coded H1-1, H1-3, H2-1, H3-1, H4-4, H5-2, H6-2, caused severe blight damage. Characteristics of the bacterial strains were Gram negative, aerobicand produced fluorescence onto King’s B medium, indicating thepathogen belongs to Pseudomonas genus. This bacterial Pseudomonas was positive of levan production and potato soft rot, which were confirmed that it wasPseudomonasmarginalis. This bacterial pathogenalso caused soft rot in post-harvest Indian pennywort. This is the first report ofP. marginalis on Indian pennywort in Vietnam.