An experiment was carried out in the experimental farm and laboratory of Can Tho University to evaluate feed utilization, nutrient digestibility, growth performance of Crossbred rabbits supplemented by catfish oil. It was a completely randomized design with 5 treatments that were 5 supplement levels of 0, 5, 7, 9, and 11 g catfish oil per rabbit per day, three replications and 4 rabbits (balanced sex) per experimental unit. The results show that the intakes of DM, CP and NDF were similar among the treatments (P>0.05), however the EE and ME intakes significantly increased when increasing supplement levels of catfish oil in the diets, and the highest values in the CFO9 and CFO11 treatments (P0.05), except for the EE digestibility that was clearly improved when  supplementing catfish oil in the diets, and the significantly highest (91.9%)