This experiment aims to evaluate the effect of pumpkin  (Cucurbita pepo) on the growth, survivals and flesh quality of white-leg shrimp in order to apply to practical production. The triplicate experiment was conducted with 4 treatments of feeding including (i) pellet feed (control), (ii) replacement of pellet with pumpkin at 10%, (iii) replacement of pellet with pumpkin at 20%, (iv) replacement of pellet with pumpkin at 30% daily.  Shrimps were cultured in 200-L tanks placed indoor applying bioflocs techniques with C/N ratio of 15/1. Water was at 15ppt in salinity and continuously aerated. Shrimp seeds with initial size of 5.1cm and 0.72g were stocked at 150 inds./m³.  After 90 days of culture, results showed that water quality parameters were in suitable ranges for normal development of shrimp. Shrimps of 11.1 – 12.5 cm and  13.6 – 19.9 g in BL and BW were obtained, of which BL and BW of shrimp in treatment replaced with 10% pumpkin (12.3 cm and 18.6 g) were not significantly different from those of the control (12.5 cm and 19.9 g). Replacing pellet with pumpkin at higher rate (20% and 30%) reduced the growth. However, survival rates and biomass of shrimp harvested were not significantly different among the treatments. Replacing higher rates of pumpkin resulted in stronger orange color of cooked shrimp and stronger flesh structure. Biochemical composition of shrimp flesh (protein, lipid, minerals) were not significantly different among the treatments. FCR of pellet feed were reduced for the treatments with pumpkin supplement compared to those of the control and the feed cost (for both pellet feed and pumpkin) of the treatment with 10% pumpkin replacement (43,871 VND/kg shrimp) was the lowest. The findings indicated very good potential for replacement of pellet feed with pumpkin (at rates of 10%) in shrimp culture for higher quality of shrimp and low feed cost.