AgNO3/Cu(NO3)2 nanofibers were succefully synthesized by an electrospinning method. The conditions of electrospinning included 8% PVP solution, feed rate of polymer solution of 0.5 mL/h, applied voltage of 20 kV, and the tip-to-collector distance of 8 cm. The nanofibers were carbonized at 300, 450, and 600 oC to obtain Ag/CuO nanofibers. The Ag/CuO nanofibers
were characterized by SEM, TEM, and XRD analyses to confirm the morphology as well as the formation of copper oxide and silver. The Ag/CuO nanofibers were used to construct a nonenzymatic glucose sensor. The Ag/CuO NFs-IGZO electrode was applied to detect glucose by cyclic voltammetry (CV). The direct oxidation of glucose in sodium hydroxide medium at Ag/CuO nanofiber modified electrodes has been investigated.