Vernonia amygdalina Del., belonging to the Asteraceae family, is a naturally occurring plant found in many parts of the world, especially suitable for hot and humid climates such as in Africa and Southeast Asian countries. Among the diverse biological activities of bitter leaf, due to the wide variety of constituent compounds, we were interested in studying the mosquito larval killing ability of this parasitic plant. The raw material used in this study was plant powder collected in Can Tho, which was extracted with methanol to obtain the alcohol extract. This alcohol extract was then sequentially extracted with organic solvents n-hexane and ethyl acetate to produce extracts of different polarities. First, the Brine-shrimp test was performed on the extract samples, that is, to examine the toxicity of the extract to small organisms such as Artemia larvae. Two of the four extracts of V. amygdalina showed good results in toxicity to Artemia salina larvae, namely the total MeOH extract and EA. These two extract mixtures showed better LC₅₀ values than some standards used in the Meyer method. Next, the ability to kill Aedes mosquito larvae through larval killing was examined, among the samples, the hexane extract showed the best toxicity. In addition, the antibacterial survey showed that the extracts of this V. amygdalina plant only weakly inhibited the tested microorganisms including Gram-negative, Gram-positive bacteria and yeasts at mg/mL concentration.