Two new phytosphingosine-type including ceramide and cerebroside (1 and 2) were isolated from n-hexane extract of the whole plant of Acanthus ilicifolius Linn. The chloroform fraction afforded three known compounds as apigenin (3) baicalein (4) and lyoniresinol (5). The structures of these phytosphingosine were elucidated based on the spectroscopic data and the chemical analyse and determined as 2S,3S,4R,5S-2-{[(2′R)-2′-hydroxy octacosanoyl]amino}heptadeca-1,3,4,5-tetraol (1), 1-O-(β-D-glucopyranosyl)-(2S,3S,4R)-2-{[hexadecanoyl]amino}untriacontene-1,3,4-triol (2),