The result showed the fertilization 90.0 kg N ha-1 had total nitrogen uptake, plant height, leaves number, concentrations of chlorophyll a, b and a+b, and sesame grain yield higher than that fertilization at 0.0 kg N ha-1, with the corresponding values of, 110.0 mg pot-1, 75.5 cm, 17.6 leaves plant-1, 10.9 μg mL-1, 2.95 μg mL-1, 13.9 μg mL-1 and 6.34 g pot-1, compared to 23.4 mg pot-1, 26.6 cm, 12.3 leaves plant-1, 8.11 μg mL-1, 1.94 μg mL-1, 10.1 μg mL-1 and 2.17 g pot-1, respectively. Supplementation of only Enterobacter asburiae ASD-07, only E. asburiae ASD-28 or both E. asburiae ASD-07 and E. asburiae ASD-28 raised the available nitrogen content in soil, the total nitrogen uptake, plant height, capsules number per plant and sesame grain yield, with the values of 4.20-6.40 mg NH4+ kg-1, 32.4-45.0 mg N pot-1, 1.7-4.2 cm, 3.75-5.15 capsules plant-1 and 1.51-1.62 g pot-1, respectively. The addition of NFRB strains either individual or dual ASD-07 and ASD-28 combined with 45.0 or 67.5 kg N ha-1 of nitrogen fertilizer resulted in a higher sesame yield, in comparison to fertilization 90.0 kg N ha-1 as a recommended fertilizer formula with no bacteria.