The study was conducted to evaluate the susceptibility of 58 Vibrio parahaemolyticus bacterial isolates to 16 antibiotics. These bacterial isolates were recovered from diseased shrimp which displayed typical pathology of AHPND such as hepatopancreatic atrophy, empty gut, and hepatopancreatic changes including hemocytic infiltration and bacterial infection. Results of antibiotic susceptibility testing by the disk diffusion method showed that single resistance to tested antibiotics was relatively rare. The bacterial strains were resistant to amoxicillin (100%), cephalexin (100%), Sulfadiazine Sodium (94.7%), and Erythromycin (87.7%). Sensitivity of tested strains was recorded with doxycycline (84.2%) and oxytetacylin (49%). The minimum inhibitory concentrations (MICs) were determined for the sensitive isolates using a broth macro dilution method. The majority of tested isolates had an MIC value of 2μg/mL with doxycycline. The current study suggests caution in the use of antibiotics for the prevention and treatment of AHPND in shrimp farming.