The aim of this study is to determine the optimal stocking density and production in larval rearing of whiteleg shrimp (Litopenaeus vannamei) applied biofloc technology (BFT). The experiment consisted of four treatments with triplicates each in different stocking densities (treatments, i.e. 150 larvae L-1 , 200 larvae L-1 , 250 larvae L-1 and 300 larvae L-1 , respectively). Larvae were stocked in 500 L-1 tanks at a salinity of 30‰ with continuous aeration. Sugar was provided daily as a carbon source to rearing tanks and supplemented from the Mysis 3 stage; C:N was manipulated at a ratio of 20:1. Results displayed the water quality, total bacteria, Vibrio spp., total bacteria count and biofloc parameters were in suitable range for larvae development. The highest length and survival rate of postlarvae-12 were found at stocking density of 150 larvae L-1 (i.e., 11.59±0.16 mm; 58.7±7.9%, respectively), and they were not significantly different (p > 0.05) from those at stocking density of 200 larvae L-1 (i.e. 11.49±0.38 mm; 55.0±3.0%, respectively). The highest production was found at stocking density of 200 larvae L-1 (i.e., 110±6 postlarvae L-1 ) and it was significantly different (p < 0.05) from the remaining treatments. In short, rearing whiteleg shrimp larvae at a density of 200 larvae L-1 under BFT presented the best practice and to be recommended for application to the whiteleg shrimp hatchery protocols.