Background and Objective: Identification of individual fruit plants is really necessary in the free trade context. Application of barcoding DNA is regarded as one of the innovations in plant sciences. This study aimed to identify four varieties of star apples: ‘Lo Ren’, butter, purple butter and purple by using some of their morphological characteristics and DNA markers. Materials and Methods: The morphological characteristics including leaves, fruits, flowers and seeds of four-star apple varieties were compared Six DNA barcodes including ITS, matK, rbcL, atpF-atpH and ycf1b were utilized for their genetic analysis. The ISSR marker with four primers (ISSR03, ISSR13, UBC841 and IISRS 3G) was then used to discriminate them. DNA sequences were aligned and clustered using Mega software and the ISSR gel images were converted to binary data and analyzed the similarity using NTSYSpc 2.1 program under the UPGMA algorithm. Results: The results showed that the color of the matured fruits was an efficient distinguishable morphological feature, while the sequences of examined regions did not differ. By analyzing PCR products, it could be inferred that ISSR03 primer played a key role in the identification of purple variety among the others and UBC841 could be used to distinguish purple butter and purple ones. Although the remaining primers (ISSR13 and IISRS 3G) could not identify individual varieties, they were capable of dividing them into two groups: ‘Lo Ren’, purple and purple butter, butter star apples. Conclusion: The findings indicated that there was a complete conservation in the DNA sequences of ITS, matK, rbcL, atpF-atpH and ycf1b regions.
Trích dẫn: Đỗ Tấn Khang, Trần Thị Thanh Khương, Nguyễn Phạm Anh Thi và Trần Thị Mỹ Duyên, 2019. Tiềm năng mở rộng ứng dụng công nghệ giải trình tự gen thế hệ mới ở Đồng bằng sông Cửu Long. Tạp chí Khoa học Trường Đại học Cần Thơ. 55(Số chuyên đề: Công nghệ Sinh học)(1): 1-13.
Khang, D.T., Vu, L.A. and Dung, T.N., 2016. Effects of extraction methods and storage conditions on histamine content in frozen tuna (Thunnus albacares). Can Tho University Journal of Science. Vol 4: 63-70.
Trích dẫn: Đỗ Tấn Khang, Hồ Duy Hạnh, Lê Xuân Thái, Trần Văn Bé Năm, Nguyễn Thị Pha và Trần Nhân Dũng, 2016. Đánh giá tính chống chịu phèn nhôm của một số giống lúa MTL (Oryza sativa L.). Tạp chí Khoa học Trường Đại học Cần Thơ. 44b: 86-95.
Tạp chí khoa học Trường Đại học Cần Thơ
Lầu 4, Nhà Điều Hành, Khu II, đường 3/2, P. Xuân Khánh, Q. Ninh Kiều, TP. Cần Thơ
Điện thoại: (0292) 3 872 157; Email: tapchidhct@ctu.edu.vn
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