Clarias gracilentus, the new species belonging to Clariidae family was found in Phu Quoc island, Kien Giang province, Vietnam by the year 2011. In order to determine the maturation and breeding capability in the captive condition, broodstock having weight of 192.1 ± 54.3g were transferred from Phu Quoc island, then reared in a recirculation system. Preliminary results showed that broodstock fed with trash fish, pellet feed (41% protein) or combination of trash fish and pellet had well adapted and reached full sexual maturity with maturation rates ranged from 66.7 to 80.0%. To induced ovulation, a series treatment with single hormone of carp pituitary gland (cPG), human chorionic gonadotropin (hCG), Ovaprim, Luteinizing hormone - Releasing hormone analogue (LHRHa) plus DOM and the combinations of cPG with Ovaprim, hCG or LHRHa were tested. Results revealed that single hormone of cPG, hCG or LHRHa plus DOM was induced final maturation and ovulation process in which single injection of cPG at the dose of 8 mg.kg^-1 of female body weight obtained a good results with 32.7±10.7% and 69.1±11.2% in fertilization and survival rates, respectively. Among treatments using combination of cPG with other hormones, fish received 2 successive injections at 8 hours interval, a preliminary dose with 2 mg cPG.kg^-1 and decisive dose with 4,000 IU hCG.kg^-1 of female body weight, gave the best results with highest fertilization (61.3±12.6%) and hatching rate (63.9±11.7%). Duration of embryonic development was about 25 hours started from fertilization occurred at water temperature of 28-30^oC. Larvae totally absorbed their yolk sac at day 9 after hatching. These preliminary results give promising prospects for domestication progress and the success of mass seed production of this species in future.  

 Keywords: Clarias gracilentus, sexual maturity, induced spawning, hormones.