Article info.

ABSTRACT

Received 26 Aug 2016
Revised 17 Nov 2016

Accepted 30 Mar 2018

Infectious bronchitis, caused by gammacoronavirus, is a high contagious disease of chickens. The disease was characterized by respiratory, renal, urogenital, and alimentary infections. Different strains of IBV may show variable tissue tropisms. For example, the classical strains of Massachussetts serotype (M41) targeted in respiratory and reproductive system while variant serotypes including QX, 793/B, D388 have been detected in respiratory, reproductive, alimentary tract and kidney. Because the evolution and mutation of IBV have contributed to the emergence of new genotypes of IBV, the outbreak of IB still occurred in the vaccinated flocks. Therefore, diagnosis of IB plays an important role for limiting the effects caused by IBV infection. Available laboratory tests for diagnosis of IBV include virus isolation, electron microscope, immunohistochemistry, serological tests (virus neutralization, haemagglutination inhibition, enzyme-linked immunosorbent assay, agar gel immunodiffusion), and genotype identification (Reverse transcription – polymerase chain reaction (RT-PCR), genome sequencing [using next generation sequencing]).

Keywords

Coronavirus, chicken, laboratory tests, Infectious bronchitis virus (IBV), pathogenesis, RT-PCR

Cited as: Bich, T.N., Khanh, N.P. and Loan, N.C., 2018. Pathogenesis of infectious bronchitis virus (IBV) and laboratory test methods available to detect IBV in chickens. Can Tho University Journal of Science. 54(2): 40-45.