ARTICLE INFO

ABSTRACT

Received date: 03/09/2015

Accepted date: 19/02/2016

In this study, crude polysaccharides were extracted from brown seaweed Sargassum flavicans by three extraction solvents including hot-water (SF1), 0.1N HCl (SF2), and 90% aqueous ethanol (SF3). The extracts were then analyzed for chemical composition and antioxidant activity. The results showed that among three extraction solvents, 0.1N HCl extract exhibited higher yield (41.1%), followed by hot-water (24.5%) and 90% aqueous ethanol solvent (12.8%). The color of these freeze-dried extracts changed from brown (SF1 and SF2) to brown green (SF3). The crude protein concentrations were 3.6; 6.1; and 5.2% for SF1, SF2, and SF3, respectively. Extract SF2 had higher concentration of total phosphorus (0.34%) than that of SF1 (0.17%). Total phlorotannin concentration of extracts was in the range of 0.27-0.47%. Also, extract SF2 had the highest percentage of sulfate content (3.5%). The free radical scavenging activity, ferrous ion chelating activity, and ferric reducing power of extracts were increased with increasing of concentrations of polysaccharides. Polysaccharide was extracted by 0.1N HCl (SF2) showed the highest antioxidant activity. These results indicated that the polysaccharide extracts of brown seaweed S. flavicans possessed a good antioxidant
activity.

KEYWORDS

Antioxidant activity, seaweed extract, polysaccharide, Sargassum flavicans