The aim of this study is to analyze factors which are influencing the development of ecotourism at Phong Dien district, Can Tho city, Vietnam, by Explorer Factor Analysis method. The questionnaires were distributed to a total of 168 local ecotourism business owners, domestic tourists, and foreign visitors at eco-destinations in statistical analyses. TheresearchrevealedthatthereweresevenfactorsthatinfluencedecotourismdevelopmentatPhongDiendistrict namely: staff factors with six variables (tour guide/staff take care of guests carefully, tour guide/staff were equipped with foreign languages and communication skills, tour guide/staff noticed guests concerns, tour guide/staff understood guests needs,tourguide/staff were equipped with professional knowledge,the behavior of tour guide/staff at souvenirs shops was polite and honest); tangible factors with four variable (stayed destination systems, mean of transportation in the orchard such as carriages, bicycles, boats, diversity souvenir products; and costume guides/staff); assurance elements with three variables (ensure food safety, appropriate prices, and security conditions); trust factors included three variables (clear information on the destinations,having books, leaflets of the destinations, and advertising services); local specialty factors with two ingredients (natural beautiful sights, traditional dishes); facility and infrastructure factors consisted of two items (convenience of transport system, modern communications system); provided factors with two variables (willingness of the guide/staff to serve visitors,cooperation of eco-destinations in order to avoid over lapping in tourism products). Thanks to the finding of study, the result will benefit local authorities, management, tourist agencies, ecotourism business owners and local people with real data analysis. This paper also proposes some solutions to increase quality of ecotourism and encourage more and more tourists to Phong Dien district, Can Tho city, Vietnam.