Rhizobacteria  in  the  acid  sulphate  soil  rhizosphere  of  rice  grown  the Mekong  Delta,  Vietnam  was  studied.  A  total  of  160  isolates  were isolated  on  two  media  (Burk’s  N-free  and  NBRIP)  from  80  soil rhizosphere  samples  were  taken  in  4  regions  (Tu  Giac  Long  Xuyen, Dong Thap Muoi, Trung Song Hau, Ban dao Ca Mau)  and all of them have  ability  of  nitrogen  fixation  and  phosphate  solubilization.  The sequences from selected rhizobacteria (14  isolates) were analysed 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (8F and 1492R) and sequenced. The results showed they had high degrees of similarity to those of the GenBank references strains(between  98%  and  99%).  From  14  isolates,  8  belonged  to  Bacilli  while  Gammaproteobacteria  was  6. There were 18/63 and 22/63 isolates producing IAA and siderophores, respectively. From these results showed that there are  two  strains as  Enterobacter asburiaeHG9-3a,  Bacillus  subtilis  HG10-3a  and  Bacillus  subtilis  CJL18  revealed  promising candidates with multiple beneficial characteristics and they have the potential for application as inoculants adapted to poor soils and local crops because they are not only famous strains but also are safety strains for agricultural sustainable, especially for  rice  cultivation on  acid sulphate soil in the future