The objective of this study is to analyze the visitor's assessment of the protection of tourism resources in the nature reserve in the Mekong Delta. The questionnaire survey method was used to collect information. Descriptive statistics and one-way analysis of variance were used to analyze the data. Research results show that tourists appreciate the protection of tourism resources in the study area; the protection of tourism resources varies among protected areas; reserves of Tra Su Melaleuca forest, Mui Ca Mau, Tram Chim protect the best tourism resources; conservation areas of Bac Lieu bird sanctuary and U Minh Thuong are not good at protecting tourism resources. Improving the livelihoods of the community; awareness raising communication among many stakeholders; actively using and exploiting natural resources; restoration and embellishment are measures contributing to the protection of tourism resources.