This study were conducted to evaluate the effects of salinity on the development and the contributions of Bacillus subtilis on the growth and reproduction of Artemia franciscana Vinh Chau. Experiment was set up with 6 treatments and were run triplicates per each with two factorial design in which salinity (30, 70 and 90‰) combined the supplementation or without B. subtilis into Artemia culture medium. Artemia were cultured at the density of 500 ind./L and were fed by commercial feed. After 15 days, Artemia obtained highest survival rate (61.5%) at 90‰ with B. subtilis supplementation. Artemia in this treatment also reached highest values in the length (8.35 mm), mating rate (51.3%) and fecundity (45 offspring/female), these results was significant difference from other treatments (pB. subtilis can survive, growth and play a positive role in water quality improvement, growth and reproduction of Artemia in culture medium at the salinity of 90‰.