A total of 319 nasal swab samples were collected to clarify the prevalence and antimicrobial susceptibility of E. coli in the respiratory tract of goats in Can Tho City, Vietnam. It indicated that E. coli was detected at a relatively minor rate (8.46%), and their prevalence in male goats and dairy goats was higher than in others. However, the ages and health conditions of goats did not affect the presence of E. coli in those goats. Those E. coli strains were still susceptible to seven examined antibiotics, but the resistance had been recorded in ampicillin (25.93%) and bactrim (25.93%) in this study. Those E. coli strains (33.33%) could resist one to seven antibiotics with several patterns; the pattern of Am + Ac (7.41%) was more common than others. Moreover, E. coli strains harbored antibiotic-resistance genes, and blaampC was detected at the highest rate (92.11%), followed by sulII (43.86%), tetA (24.56%), and qnrA (5.26%). Of those E. coli strains, 22.81% showed multi-harbor from two to four examined genes with several patterns of antibiotic-resistance genes; the most detected pattern was blaampC + sulII + tetA (7.89%). Thus, controlling the prevalence of antibiotic-resistant E. coli in the respiratory tract of goats will protect animal and public health.