Background and Objective: Perilla frutescens (Lamiaceae family) is an annual herbaceous species of the mint family originating from East Asia. This study aimed to evaluate some biological activities of Perilla frutescens extracts and essential oils collected at three locations, Can Tho City, An Giang and Vinh Long Province. Materials and Methods: Fresh perilla plants were collected from perilla farmers in Can Tho City (CT), An Giang (AG) and Vinh Long (VL) provinces. Samples were extracted using ethanol as solvent and isolated essential oil. Antioxidant activity was evaluated by two assays DPPH and ABTS. The antibacterial activity was conducted on Escherichia coli, Staphylococcus aureus and Propionibacterium acnes by disc diffusion method. Results: Quantitative results of total polyphenols and total flavonoids showed that the Can Tho treatment was the highest with 12.78 mg GAE/g extract and 65.99 mg QE/g extract. Essential oils contain a total of 38 volatile compounds. The major predominant compounds were perillaldehyde, 2-Furyl n-pentyl ketone, $-Caryophyllene, $-Linalool, valeric acid pent-2-en-4-ynyl ester and limonene in variable levels. Conclusion: All extracts and essential oils of P. frutescens leaves have revealed potent antioxidants and antibacterial activities. The different ecological factors determine the content and various biological activities