Ehretia asperula Zoll. & Moritzi was a common medicinal herb found in
several Asian nations. This herb was found in many provinces in northern Vietnam,
especially Hoa Binh province. Leaves of E. asperula cultivated in Tay Ninh Province were
used to isolate compounds and evaluate their potential as antioxidants by five methods,
including TAC, RP, FRAP, DPPH, and ABTS•+. Seven compounds were isolated and
elucidated from the ethyl acetate fraction, including kaempferol (1), astragalin (2),
nicotiflorin (3), rutin (4), caffeic acid (5), (-)-loliolide (6), and daucosterol (7), where
compounds 1, 4, 6, and 7 were found from this species for the first time. All isolated
compounds from E. asperula leaves exhibited antioxidant activity, consisting of
5.70 ± 0.01 to 299.13 ± 10.19 μg/mL for EC50 values. Especially compound 5 had a very
strong antioxidant effect based on five methods TAC (EC50 = 8.43 ± 0.15 μg/mL), RP
(EC50 = 6.79 ± 003 μg/mL), FRAP (EC50 = 12.72 ± 006 μg/mL), DPPH
(EC50 = 5.57 ± 0.02 μg/mL), and ABTS•+ (EC50 = 5.70 ± 0.01 μg/mL). From our results,
Tay Ninh Province's E. asperula leaves are abundant in naturally occurring antioxidants,
indicating their potential use as therapeutic materials