Folium sauropi is grown commonly in Vietnam, it is a vegetable that contains many bio-active compounds such as chlorophyll, polyphenol, and antioxidant activity. The use of natural pigments having clear origin is a trend in food processing nowadays. The objective of this study was to find suitable conditions for extracting chlorophyll with antioxidant activity (DPPH) in folium sauropi leaves by microwave-assisted extraction. When extracted with acetone of 90°, microwave power of 300W, solid to solvent ratio of 1:30, microwave-assisted extraction time of 120 seconds; chlorophyll content (14.43± 0.16 μg/mL), and DPPH radical-scavenging activity (886.64± 15.89 μmol/L) obtained highest.