The study was carried out to assess the effects of additives (glycerol and sorbitol) and the role of supplement rate of ascorbic acid on reducing lipid and protein oxidation in dried snakehead fish (Channa striata). The research results revealed that adding additives increased the amount of dry matter of the material, leading to a shorter drying process. The addition of glycerol and sorbitol at different ratios were the causes of the change of drying time and sulfhydryl index, peroxide value, TBARS index, salt content, color, and water activity level of the dried snakehead fish. Using 3% glycerol combined with 4% sorbitol, the drying time was 26.21 hrs. Dried snakehead fish product had a salt content of 6.04%, a water activity of 0.576, a brightness of 43.90, a peroxide value of 0.064 meq/kg lipid, a TBARS of 4.40 mg MDA/kg, a total sulfhydryl of 30.22 µmol/g protein, and an available sulfhydryl of 11.41 µmol/g protein. When ascorbic acid was added, the oxidation indexes of dried snakehead fish were significantly reduced; however, ascorbic acid showed a superiority at 0.04% with peroxide indices of 0.04 mEq/kg lipid, TBARS was 4.28 mg MDA/kg a total sulfhydryl of 31.60 µmol/g protein and sulfhydryl available reached to 11.94 µmol/g protein. The difference between the experiment of adding ascorbic acid was even more significant after four weeks of storage when the dried snakehead fish was less lipid and protein oxidation.